--- /dev/null
+[[!meta title="Characterization of Agrobacterium tumefaciens DNA ligases C and D."]]
+[[!tag enzyme ligase]]
+
+Zhu H and Shuman S.
+
+Nucleic Acids Res. 2007;35(11):3631-45 doi:10.1093/nar/gkm145
+
+Characterization of Agrobacterium tumefaciens DNA ligases C and D.
+
+[[!pmid 17488851 desc="In presence of Co²⁺, can extend an oligonucleotide on a template using rNTPs."]]
17405768 [microfluidics]
Cycling 100 times (94°C for 30 s, 55°C for 30 s, and 72°C for 60 s) in 0.1% DDM, x1 Pfu Buffer before use improves product yield.
-17488851 [enzymes]
-In presence of Co²⁺, can extend an oligonucleotide on a template using rNTPs.
-
17488852 [enzymes]
Useful to prepare 2' phosphate ends, which are substrate for the yeast tRNA ligase.