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+[[!meta title="Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR."]]
+[[!tag amplification PCR]]
+
+Salk JJ, Sanchez JA, Pierce KE, Rice JE, Soares KC, Wangh LJ.
+
+Anal Biochem. 2006 Jun 1;353(1):124-32 doi:10.1016/j.ab.2006.02.012
+
+Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR.
+
+[[!pmid 16540077 desc="The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task."]]
16527264 [enhancers]
Not read. An enhancer of Rpx/Hesx1 conserved between mouse and xenopus.
-16540077 [misc]
-The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task.
-
9380524 [protocols]
Concentrations as high as 1 M or 2.5 M can strongly improve the yield.