Old

diff --git a/biblio/16461948.mdwn b/biblio/16461948.mdwn
new file mode 100644 (file)
index 0000000..91c86d6
--- /dev/null
+++ b/biblio/16461948.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein."]]
+[[!tag reverse_transcription]]
+
+J Biomol Tech. 2005 Sep;16(3):239-47
+
+Piché C, Schernthaner JP.
+
+Optimization of in vitro transcription and full-length cDNA synthesis using the T4 bacteriophage gene 32 protein.
+
+[[!pmid 16461948 desc="T4gp32 increases the yield of the smallest cDNAs by 10 %."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index 833287f07bfd582785313659901f4bb663efa5a8..aaca0a6b95b1831f581ba3ddaedad57cb033d8b4 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -661,9 +661,6 @@ Not read. An enhancer of Rpx/Hesx1 conserved between mouse and xenopus.
 16540077 [misc]
 The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task.
 
-16461948 [protocols]
-T4gp32 increases the yield of the smallest cDNAs by 10 %.
-
 2326177 [protocols]
 More inert than glycogen.
 

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 04109217ed70b4e71fe681193dc23d43fee5b863..2f8af9a5acbfd9a7aaf386357dace1529d1e4e0c 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -2,6 +2,10 @@
 
 (redaction in progress)
 
+Additives that increase reaction performance:
+
+ - T4 bacteriophage gene 32 protein (T4gp32, [[Piché _et al._, 2005|biblio/16461948]]).
+
 Reverse-transcriptases have a DNA-dependent DNA polymerase activity.
  - It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.
  - It is also a source of antisense artefacts when the RT makes a second-strand cDNA