Expand.

diff --git a/biblio/11713326.mdwn b/biblio/11713326.mdwn
index 1a698d93a318ba17b3936fd0ea8403b9206393c6..7a677af16cac226e9f825806605cf88f2d94b7c1 100644 (file)
--- a/biblio/11713326.mdwn
+++ b/biblio/11713326.mdwn
@@ -1,3 +1,10 @@
 [[!meta title="Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach."]]
 [[!tag cap method]]
+
+Efimov VA, Chakhmakhcheva OG, Archdeacon J, Fernandez JM, Fedorkin ON, Dorokhov YL, Atabekov JG.Efimov VA1, Chakhmakhcheva OG, Archdeacon J, Fernandez JM, Fedorkin ON, Dorokhov YL, Atabekov JG.
+
+Nucleic Acids Res. 2001 Nov 15;29(22):4751-9.
+
+Detection of the 5'-cap structure of messenger RNAs with the use of the cap-jumping approach.
+
 [[!pmid 11713326 desc="Binds an oligonucleotide to the cap using its free diol group, and integrates its sequence to the full-length cDNA using template-switching."]]

diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index 9a0c954a240453944fcf510eebd3ea851a380852..206f83284ae86b27a3494753ae462ef25593787b 100644 (file)
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -16,6 +16,11 @@ Methods for enriching capped RNAs.
    the first-strand cDNA, ligate a double-stranded adapter ending with a
    single-strand (T)TTTGGG overhang, and prime second-strand synthesis.
 
+ - Cap-jumping ([[Efimov et al., 2001|biblio/11713326]]): oxidize diols,
+   covalently bind a 3′-amine oligonucleotide.  The [[reverse_transcriptase]]
+   ireads through the cap structure and chemical bond, and integrates the reverse
+   complement of the oligonucleotide to the first-strand cDNA.
+
  - [[Cleptet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4
    DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4
    RNA ligase and a single-stranded linker.