pubmed

diff --git a/biblio/10.1101_2020.01.16.905182.mdwn b/biblio/10.1101_2020.01.16.905182.mdwn
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-[[!meta title="Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq"]]
-[[!tag bioRxiv promoter method]]
-
-bioRxiv Posted January 16, 2020 doi:10.1101/2020.01.16.905182 
-
-Robert A. Policastro, R. Taylor Raborn, Volker P. Brendel and Gabriel E. Zentner
-
-Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq
-
-[[!doi 10.1101/2020.01.16.905182 desc="Terminator-treated RNA reverse-transcribed with random N5 primers.  The (biotinylated) TSO is added in the last 5 min of the RT (~40 µM final).  The TSO carries a P5 linker and the RTP a P7 (indexed) linker.  Follows a standard PCR and sequencing.  The YR motif is detected in yeast and K562 cells.  High amounts of rRNA remains in yeast"]]

diff --git a/biblio/32660958.mdwn b/biblio/32660958.mdwn
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+++ b/biblio/32660958.mdwn
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+[[!meta title="Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq"]]
+[[!tag promoter method]]
+
+Robert A. Policastro, R. Taylor Raborn, Volker P. Brendel and Gabriel E. Zentner
+
+Genome Res. 2020 Jun;30(6):910-923. doi:10.1101/gr.261545.120
+
+Simple and efficient measurement of transcription initiation and transcript levels with STRIPE-seq
+
+[[!pmid 32660958 desc="Terminator-treated RNA reverse-transcribed with random N5 primers.  The (biotinylated) TSO is added in the last 5 min of the RT (~40 µM final).  The TSO carries a P5 linker and the RTP a P7 (indexed) linker.  Follows a standard PCR and sequencing.  The YR motif is detected in yeast and K562 cells.  High amounts of rRNA remains in yeast"]]

diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn
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--- a/tags/template_switching.mdwn
+++ b/tags/template_switching.mdwn
@@ -55,6 +55,10 @@
    ~15% of the 5′ end alignments have extra Gs, but the genomic distribution is
    bimodal.  Peaks with significant amounts of "extra G" nucleotides are marked as TSS.
 
+ - [[Policastro and coll, 2020|bilbio/32660958]] and others before them add the
+   template-switching oligonucleotide after the reverse-transription has been
+   incubated for some time.
+
 ### Effect of chemical composition of the TS oligonucleotide
 
 Originally, the TSOs were all-RNA.  Since this is expensive to synthesise,