Café

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+[[!meta title="Germline development during embryogenesis of the larvacean, Oikopleura dioica."]]
+[[!tag Oikopleura H3S28p]]
+
+Dev Biol. 2022 Jan;481:188-200. doi:10.1016/j.ydbio.2021.10.009
+
+Nishida H, Matsuo M, Konishi S, Ohno N, Manni L, Onuma TA.
+
+Germline development during embryogenesis of the larvacean, _Oikopleura dioica_.
+
+[[!pmid 34755656 desc="Identified a CAB (centrosome-attracting body) “for the following reasons. (1) There was a clear boundary between the CAB-like region and the general cytoplasm, but the membrane structure did not surround the CAB. (2) It contained an electron-dense matrix that resembled a germplasm. (3) The region was devoid of mitochondria but contained ER. (4) It was present beneath the cell membrane, and the cell surface exhibited microvilli. (5) It was present in the germline lineage cells.”  ”The longest diameter of the CAB was approximately 10 ​μm.“  It was observed between the late 8-cell stage and some 1.5-h embryos, but not after 2h.  It was stained by the H3S28p rat monoclonal antibody (Abcam ab10543) but not by DAPI.  The _snail_ mRNA also colocalises with the CAB's H3S28p staining.  A germ body (GB) “appeared [in PGCs] 13 ​min after the 5th cleavage [and] disappeared 1.5 ​h after fertilization at 20 ​°C.  It is discussed whether the GB and the CAB are or are not the same structure."]]

diff --git a/tags/H3S28p.mdwn b/tags/H3S28p.mdwn
index 7d6c92a27869dbdace1b2aabaa6fa88da2a655f1..f906bf6c2e6e9a5a660240283303f9b04414f607 100644 (file)
--- a/tags/H3S28p.mdwn
+++ b/tags/H3S28p.mdwn
@@ -40,4 +40,8 @@ punctate centromere staining at ”late prophase“, a stronger signal (but hard
 to resolve) at metaphase, a weaker signal at anaphase and a weaker or no signal
 at telophase.  Table S1, listing the antibodies used, is missing.
 
+The rat monoclonal antibody (Abcam ab10543) stains the centromere-attracting
+body in _O. dioica_, Osaka lab. strain. ([[Nishida and coll.,
+2021|biblio/34755656]]), probably by cross-reactivity.
+
 [[!inline pages="tagged(H3S28p)" limit=0]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index e4e9fcb02441adc1b8bfdd8616c20d59c155b58c..a05e4d9f4ed182cdcdbed632dac3cb551528e6cf 100644 (file)
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -397,6 +397,9 @@ Development
    hindbrain, and spinal cord, but not the midbrain.  No expression of
    _pax2/5/8_ is detected between the _otxa_ + _otxb_ and the _hox1_ territories.
    ([[Cañestro et al., 2005|biblio/16111672]]).
+ - A centromere-attracting body is resoponsible for asymetric cell division in
+   primordial germ cells ([[Nishida and coll., 2021|biblio/34755656]]).  The
+   _snail_ mRNA co-localises with it.
  - The _pum1_ and _vas4_ RNAs show localised expression during development. Prior
    hatching, _pum1_ is found outside the embryo ([[Olsen et al., 2018|biblio/29486709]]).
  - Duplicated developmental genes were found by [[Denoeud et al., 2010|biblio/21097902]],