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+[[!meta title="T4 RNA ligase: substrate chain length requirements."]]
+[[!tag ligase]]
+
+FEBS Lett. 1974 Sep 15;46(1):271-5.
+
+Kaufmann G, Klein T, Littauer UZ.
+
+T4 RNA ligase: substrate chain length requirements.
+
+[[!pmid 4609429 desc="For circularisation, (pA)8 is the shortest substrate, and the reaction is most efficient with (pA)10. The ligation efficicency then decreases with substrate length."]]

diff --git a/biblio/To_Do b/biblio/To_Do
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--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -353,9 +353,6 @@ Out of 126 ultraconserved elements in a melanogaster / A. Gambiae comparison, on
 7082652 [enzymes]
 T4RNL1 & AMP can  remove or exchange 3' pNp from RNA molecles.
 
-4609429 [enzymes]
-(pA)8 is the smallet substrate, and the reaction is most efficient with (pA)10. The ligation efficicency then decreas with substrate length.
-
 12429865 [tags]
 Provides an easy access to various statistical tests.
 

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 Work in progress.
 
+On T4 RNL1:
+
+ - Using poly-A as substrate for circularisation, minimal length is 8, optimal
+   is 10, and then efficiency decreases slowly [[Kaufmann, Klein & Littauer, 1974|biblio/4609429]].
+
 On T4 RNL2:
 
  - Primary paper: [[Ho et al., 2002|biblio/12228725]].