Caps

diff --git a/biblio/32103016.mdwn b/biblio/32103016.mdwn
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+[[!meta title="Dinucleoside polyphosphates act as 5'-RNA caps in bacteria."]]
+[[!tag cap]]
+
+Hudeček O, Benoni R, Reyes-Gutierrez PE, Culka M, Šanderová H, Hubálek M, Rulíšek L, Cvačka J, Krásný L, Cahová H. 
+
+Nat Commun. 2020 Feb 26;11(1):1052. doi:10.1038/s41467-020-14896-8
+
+Dinucleoside polyphosphates act as 5'-RNA caps in bacteria.
+
+[[!pmid 32103016 desc="T7 RNAP and E. coli RNAP efficiently initiate
+transcription with N(p)nN cap analogs at mM concentrations.  LC-MS analysis of
+sRNAs digested with Nuclease P1 detects N(p)nN caps.  Existence of the internal
+polyphosphate chain demonstrated by the fact that the N(p)nN caps are not
+fragmented by ionization, like GppppG and unlike pppGpG.  m7Gp4Gm and m6Ap3A
+were further identified using synthethic standards.  The RppH and ApaH enzymes
+cleaved caps, leaving 5′-p or 5′-ppp ends respectively.  Cap methylation
+protects from RppH cleavage but not from ApaH."]]

diff --git a/tags/cap.mdwn b/tags/cap.mdwn
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--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -69,4 +69,7 @@ Atypical caps (work in progress)
  - 5′-phospho-ADP-ribosylated RNA/DNA cap, synthethysed by RNA
    2′-phosphotransferase Tpt1 ([[Munir, Banerjee and Shuman, 2018|biblio/30202863]]).
 
+ - Mass spectroscopy and molecular biology detected dinucleoside polyphosphate caps
+   in bacteria ([[Hudeček and coll., 2020|biblio/32103016]]).
+
 [[!inline pages="tagged(cap)" limit=0]]