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+[[!meta title="Predicting Genes in Single Genomes with AUGUSTUS."]]
+[[!tag genome annotation software]]
+
+Hoff KJ, Stanke M.
+
+Curr Protoc Bioinformatics. 2019 Mar;65(1):e57. doi:10.1002/cpbi.57
+
+Predicting Genes in Single Genomes with AUGUSTUS.
+
+[[!pmid 30466165 desc="Explain how to train AUGUSTUS for new species using transcriptome data."]]
BUSCO ([[Simão and coll., 2015|biblio/26059717]], [[Waterhouse and coll.,
2017|biblio/29220515]]) assesses the presence of evolutionary conserved single-copy
-genes in the assemblies.
+genes in the assemblies. Seppey, Manni and Zdobnov EM ([[2019|biblio/31020564]])
+wrote a good introduction in _Methods Mol Biol_.
+
+BUSCO uses AUGUSTUS, and AUGUSTUS can be trained for a new species with
+transcriptome data, as explained by [[Hoff and Stanke, 2018|biblio/30466165]].
A reference assembly can be used to search for structural variants in a different
individual, for instance with NanoSV ([[Cretu and coll., 2017|biblio/29109544]]).