Un papier, un café.

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+[[!meta title="Mosaic copy number variation in human neurons."]]
+[[!tag single_cell genome human]]
+
+McConnell MJ, Lindberg MR, Brennand KJ, Piper JC, Voet T, Cowing-Zitron C, Shumilina S, Lasken RS, Vermeesch JR, Hall IM, Gage FH.
+
+Science. 2013 Nov 1;342(6158):632-7. doi: 10.1126/science.1243472
+
+Mosaic copy number variation in human neurons.
+
+[[!pmid 24179226 desc="Signal averaged over hundreds of kilobases to prevent from artefactual lapses of coverage."]]

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@@ -52,9 +52,6 @@ Aminoallyl UTP to labal cRNAs. In contrast to cDNAs, DMSO is required for subseq
 11955710 [visual cortex]
 Cat visual cortex cDNAs hybridized to human macroarrays to study critical period mechanism
 
-12636919 [single cell]
-Single cell PCR, using 5'poly1-tagged cDNAs.
-
 11222780 [amplification]
 T7 pol generates aberrant template-unrelated products. This is fixed by reducing the concentration of oligo dT primers, and of enzymes. Under these conditions, 2 rounds of RNA linear amplification is not generatng too much bias. Small-volume protocol. T4gp32, a single stranded protein, increases RT processivity.