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+[[!meta title="Second-strand cDNA synthesis with E. coli DNA polymerase I and RNase H: the fate of information at the mRNA 5' terminus and the effect of E. coli DNA ligase."]]
+[[!tag cDNA method]]
+
+D'Alessio JM, Gerard GF.
+
+Nucleic Acids Res. 1988 Mar 25;16(5):1999-2014.
+
+Second-strand cDNA synthesis with E. coli DNA polymerase I and RNase H: the fate of information at the mRNA 5' terminus and the effect of E. coli DNA ligase.
+
+[[!pmid 2833725 desc="RNAse H (also provided by the reverse-transcriptase) and ligase are not strictly necessary during second-strand synthesis."]]