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+[[!meta title="Protocol for the isolation, culture, and transfection of squid primary cells"]]
+[[!tag cell_culture]]
+
+Kim Y, Tanner HM, Rosenthal JJC, Brangwynne CP
+
+STAR Protoc. 2025 Sep 19;6(3):103994. doi:10.1016/j.xpro.2025.103994
+
+Protocol for the isolation, culture, and transfection of squid primary cells
+
+[[!pmid 40773350 desc="Fibroblast-like cells migrate out of optic lobe, eye or gill explants after trypsin digestion, and can be transfected, but no cell division was observed.  Insulin-transferrin-selenium (ITS-G), FGF and EGF are present in the cell culture medium."]]

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@@ -10,5 +10,6 @@ A few notes that just scratch the surface of a vast field…
  - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.
  - Shark cells need high osmolarity and a supplement of 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide ([[Uno and coll., 2020|biblio/33159152]]).
  - RasV12 could immortalise _Drosophila_ cells ([[Simcox and coll. 2008|biblio/18670627]]).  Such lines seem to preferentially originate from adult muscle precursor cells ([[Dequéant and coll., 2015|biblio/26438832]]).
+ - Primary squid cell culture from explants in ITS-G, FGF and EGF ([[Kai and coll., 2025|biblio/40773350]]).
 
 [[!inline pages="tagged(cell_culture)" limit=0]]