--- /dev/null
+[[!meta title="Global amplification of cDNA from limiting amounts of tissue. An improved method for gene cloning and analysis"]]
+[[!tag RACE ligase amplification protocol]]
+
+Mol Biotechnol. 2002 Nov;22(3):223-30 doi:10.1385/MB:22:3:223
+
+Reddy MK, Nair S, Sopory SK.
+
+Global amplification of cDNA from limiting amounts of tissue. An improved method for gene cloning and analysis.
+
+[[!pmid 12448877 desc="Ligating a DNA adaptor 3′ to the FScDNA using T4 RNA ligase."]]
16540077 [misc]
The PPi produced during the PCR can be removed with the pyrosequencing enzymes themselves. The antarctic phosphatase from NEB was not very efficient at this task.
-12448877 [protocols]
-Ligating a DNA adaptor 3' to the FScDNA using T4RNA ligase.
-
9380524 [protocols]
Concentrations as high as 1 M or 2.5 M can strongly improve the yield.
[[!meta title="pages tagged RACE"]]
-[[!inline pages="tagged(RACE)" actions="no" archive="yes"
-feedshow=10]]
+[[!inline pages="tagged(RACE)" limit=0]]