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+[[!meta title="RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2."]]
+[[!tag enzyme ligase]]
+
+J Biol Chem. 2004 Jul 23;279(30):31337-47. doi:10.1074/jbc.M402394200
+
+Nandakumar J, Ho CK, Lima CD, Shuman S.
+
+RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2.
+
+[[!pmid 15084599 desc="Can ligate RNA at a 3'-OH/5'-P nick in a RNA/RNA or RNA/DNA duplex."]]

diff --git a/biblio/15851476.mdwn b/biblio/15851476.mdwn
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+[[!meta title="Dual mechanisms whereby a broken RNA end assists the catalysis of its repair by T4 RNA ligase 2."]]
+[[!tag enzyme ligase]]
+
+Nandakumar J, Shuman S.
+
+J Biol Chem. 2005 Jun 24;280(25):23484-9. doi:10.1074/jbc.M500831200
+
+Dual mechanisms whereby a broken RNA end assists the catalysis of its repair by T4 RNA ligase 2.
+
+[[!pmid 15851476 desc="Complete Appylation by RNL2 is possible in absence of Mg2+, but is slower."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index bf358a97c66fc6a842a686b6444776665dd27671..d678dcf1f93bee71ed7772098481c011806a7a57 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -332,9 +332,6 @@ Alternative promoters which transcribe variants with different translational eff
 15342556 [genome]
 These blocks contain most validated and predicted transcription factor binding sites.
 
-15084599 [enzymes]
-Further characterisation of the enzyme.
-
 15509747 [visual cortex]
 Another slightly different critical period, and another PV:GFP transgene.
 
@@ -977,9 +974,6 @@ Breadth-first search: organises a network in a layered hierarchy. Highest nodes
 16893951 [amplification]
 Combined use of T7 polymerase and helicase, strong strand displacement, and branched rolling circle isothermal amplification.
 
-15084599 [enzymes]
-Complete Appylation by RNL2 is possible in absence of Mg2+, but is slower.
-
 16964210 [amplification]
 Apparently, DNA - RNA hybridisation is very tolerant to mismatches.