[[!meta title="Amplification of complex gene libraries by emulsion PCR."]]
-[[!tag emulsion]]
+[[!tag emulsion PCR]]
+
+Nat Methods. 2006 Jul;3(7):545-50 doi:10.1038/nmeth896
+
+Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD.
+
+Amplification of complex gene libraries by emulsion PCR.
[[!pmid 16791213 desc="Protects the templates frome size bias by isolating them from each other."]]
[[!meta title="BEAMing: single-molecule PCR on microparticles in water-in-oil emulsion."]]
[[!tag emulsion biotin]]
+
+Nat Methods. 2006 Jul;3(7):551-9 doi:10.1038/nmeth898
+
+Diehl F, Li M, He Y, Kinzler KW, Vogelstein B, Dressman D
+
+BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions.
+
[[!pmid 16791214 desc="Recommends (in Box 1) a double-biotin to avoid breaking the complexes at high temperatures."]]
[[!meta title="Directed evolution by in vitro compartmentalization."]]
[[!tag emulsion]]
+
+Nat Methods. 2006 Jul;3(7):561-70 doi:10.1038/nmeth897
+
+Miller OJ, Bernath K, Agresti JJ, Amitai G, Kelly BT, Mastrobattista E, Taly V, Magdassi S, Tawfik DS, Griffiths AD.
+
+Directed evolution by in vitro compartmentalization.
+
[[!pmid 16791215 desc="Method for water in oil in water emulsion (w/o/w)."]]
- In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%).
- In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%).
+ - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in presence of high salt (100 mM NaCl), SDS (1%), TritonX-100 (1%) (oil: mineral, superfactant: ABIL WE09).
[[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]