Au café.

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+[[!meta title="Digital encoding of cellular mRNAs enabling precise and absolute gene expression measurement by single-molecule counting."]]
+[[!tag transcript_count template_switching fingerprint method single_cell]]
+
+Fu GK, Wilhelmy J, Stern D, Fan HC, Fodor SP.
+
+Anal Chem. 2014 Mar 18;86(6):2867-70. doi:10.1021/ac500459p
+
+Digital encoding of cellular mRNAs enabling precise and absolute gene expression measurement by single-molecule counting.
+
+[[!pmid 24579851 desc="only 14 to 23 % of the cDNAs were template-switched."]]