Medium optimisation.

diff --git a/biblio/12474249.mdwn b/biblio/12474249.mdwn
index 33f74698477ad5fc148815cc067c55aaf602a1e4..0b1b128ec79f1aa237edc8cf8a0a19d7aaac1883 100644 (file)
--- a/biblio/12474249.mdwn
+++ b/biblio/12474249.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Optimization of a feed medium for fed-batch culture of insect cells using a genetic algorithm"]]
-[[!tag method machine_learning cell_culture]]
+[[!tag method machine_learning cell_culture cell_line]]
 
 Marteijn RC, Jurrius O, Dhont J, de Gooijer CD, Tramper J, Martens DE.
 

diff --git a/biblio/30747414.mdwn b/biblio/30747414.mdwn
new file mode 100644 (file)
index 0000000..dbb43ef
--- /dev/null
+++ b/biblio/30747414.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Genetic algorithm as an optimization tool for the development of sponge cell culture media"]]
+[[!tag cell_culture machine_learning]]
+
+In Vitro Cell Dev Biol Anim. 2019 Mar;55(3):149-158. doi:10.1007/s11626-018-00317-0
+
+Munroe S, Sandoval K, Martens DE, Sipkema D, Pomponi SA.
+
+Genetic algorithm as an optimization tool for the development of sponge cell culture media
+
+[[!pmid 30747414 desc="4 generations of 30 experiments each screening the concentrations of all amino acids."]]

diff --git a/tags/cell_line.mdwn b/tags/cell_line.mdwn
index b3df749e800d176457c9a8030856c87d2eb3b3f8..9a073fbd6aa315077f868083c0c268f223cbacfc 100644 (file)
--- a/tags/cell_line.mdwn
+++ b/tags/cell_line.mdwn
@@ -4,6 +4,8 @@ A few notes that just scratch the surface of a vast field…
 
  - [[Echalier and Ohanessian (1969)|biblio/4976834]] reported a culture of _Drosophila_ cells that spontaneously transformed.
  - [[Schneider's (1972)|biblio/4625067]] report of the establishment of the S2 cell line.
- - [[Kawamura and coll (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.
+ - [[Marteijn and coll. (2003)|biblio/12474249]] optimised a culture medium using a genetic algorithm.  This methdod
+   has been used at least once in invertebrates ([[Munroe and coll. (2019)|biblio/30747414]]).
+ - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.
 
 [[!inline pages="tagged(cell_line)" limit=0]]