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diff --git a/biblio/31543449.mdwn b/biblio/31543449.mdwn
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+[[!meta title="Evolution of the U2 Spliceosome for Processing Numerous and Highly Diverse Non-canonical Introns in the Chordate Fritillaria borealis."]]
+[[!tag Oikopleura intron]]
+
+Curr Biol. 2019 Oct 7;29(19):3193-3199.e4. doi:10.1016/j.cub.2019.07.092
+
+Henriet S, Colom Sanmartí B, Sumic S, Chourrout D.
+
+Evolution of the U2 Spliceosome for Processing Numerous and Highly Diverse Non-canonical Introns in the Chordate Fritillaria borealis.
+
+[[!pmid 31543449 desc="In Fritillaria borealis, most ancestral canonical
+introns were lost.  New introns with non-canonical splice sites were created by
+the insertion of MITEs (miniature inverted-repeat transposable elements) after
+TA sites.  Splicing of non-canonical introns can be inhibited with Pladienolide
+B.  Non-canonical introns of F. bor and O. dioica genes can not be spliced in
+HEK293T cells, but canonical introns can.  In ~90% of lariats from F. bor or O.
+dio, the branchpoint is an A.  Distance to 3′ splice site is similar in both
+species.  A single U2 spliceosome was found in F. bor."]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 752a59d6191cdddd849876f58f8904f2d05cab42..1259ee44fc61723daad21d8d037c43c38b10abe4 100644 (file)
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -255,12 +255,17 @@ Transcriptome
  - “Maternal promoters in O. dioica were located on the X-chromosome more
    frequently than expected” ([[Danks et al, 2018|biblio/29482522]]).
  - Introns are very small (peak at 47 base pairs, 2.4% > 1 kb, [[Denoeud et
-   al., 2010|biblio/21097902]]) and subjected to a large turnover: many ancestral
-   introns are lost, and many new species-specific introns found ([[Edvarsen et
-   al., 2004|biblio/15638456]]).  Introns are gained by insertion of transposon-like elements and
-   by reverse splicing, ([[Denoeud et al., 2010|biblio/21097902]]).  Larger introns tend
-   to be older, and among the large introns, the older contain repeat elements less frequently
-   than the newer ([[Denoeud et al., 2010|biblio/21097902]]). 
+   al., 2010|biblio/21097902]]) and subjected to a large turnover: many
+   ancestral introns are lost, and many new species-specific introns found
+   ([[Edvarsen et al., 2004|biblio/15638456]]).  Introns are gained by insertion
+   of transposon-like elements and by reverse splicing, ([[Denoeud et al.,
+   2010|biblio/21097902]]).  Larger introns tend to be older, and among the large
+   introns, the older contain repeat elements less frequently than the newer
+   ([[Denoeud et al., 2010|biblio/21097902]]).  In _Fritilaria borealis_, most
+   introns are derived from the insertion of MITE elements and are non-canonical
+   ([[Henriet and coll., 2019|biblio/31543449]]).
+ - HEK293T cells can only splice canonical _O. dioica_ (and _F. borealis_) introns,
+   but not the non-canonical ones ([[Henriet and coll., 2019|biblio/31543449]]).
  - 12 developmental stages were studied with tiling arrays by [[Danks et al., 2013|biblio/23185044]].
  - On the histone mRNAs, no purine-rich histone downstream element (HDE) was found
    by [[Chioda, Eskeland and Thompson in 2002|biblio/12446815]].