[[!meta title="PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets."]]
-[[!tag emulsion]]
+[[!tag emulsion PCR]]
+
+Nucleic Acids Res. 2005 Oct 6;33(17):e150 doi:10.1093/nar/gni143
+
+Kojima T, Takei Y, Ohtsuka M, Kawarasaki Y, Yamane T, Nakano H.
+
+PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets.
+
[[!pmid 16214800 desc="The composition of the mixture has been modified so that it stays stable in a PCR of 55 cycles of 10 minutes. Uses Sun Soft N° 818SK."]]
- In [[Nakano _et al._, 2005|biblio/16233792]], by centrifugation (oil: silicone; superfactant: TritonX-100 0.1%).
- In [[Agresti et al., 2005|biblio/16260754]], by centrifugation in presence of diethyl ether (oil: mineral; superfactant: TritonX-100 0.1% and Span 80 4.5%).
- - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in presence of high salt (100 mM NaCl), SDS (1%), TritonX-100 (1%) (oil: mineral, superfactant: ABIL WE09).
+ - High salt:
+ - In [[Dielh _et al._, 2006|biblio/16791214]], by pipetting up and down in 100 mM NaCl, 1 % SDS, 1 % TritonX-100 and Tris (oil: mineral, superfactant: ABIL WE09).
+ - In [[Kojima _et al._, 2005|biblio|16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK).
+
+Different kinds of superfactants: TritonX-100, Span 80, Tween 80 (many articles), ABIL WE09 ([[Dielh _et al._, 2006|biblio/16791214]] and others), Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid [[Kojima _et al._, 2005|biblio|16214800]]).
[[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]