Polyamines

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+[[!meta title="An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines."]]
+[[!tag cell_culture method screen]]
+
+Burnette M, Brito-Robinson T, Li J, Zartman J.
+
+Mol Biosyst. 2014 Oct;10(10):2713-23. doi:10.1039/c4mb00155a
+
+An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines.
+
+[[!pmid 25096480 desc="“Using this approach we developed an intermediate “ZO Fortified” medium, made up of ZO media supplemented with insulin (5 μg mL−1), trehalose (26.4 mM), l-alanyl-l-glutamine (ala-gln, 12 μM), and l-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (A2P, 0.08 μM), with pH adjusted to 6.75.”  410 compounds were screened.  “All of the repeated positive hits were polyamines (spermine, spermidine, and putrescine)”"]]

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@@ -4,7 +4,7 @@ A few notes that just scratch the surface of a vast field…
 
  - [[Echalier and Ohanessian (1969)|biblio/4976834]] reported a culture of _Drosophila_ cells that spontaneously transformed.
  - [[Schneider's (1972)|biblio/4625067]] report of the establishment of the S2 cell line.
- - Polyamines are needed for the long-term passaging of some cell lines ([[Mitsuhashi 1998|biblio/9769143]]).
+ - Polyamines are needed for the long-term passaging of some cell lines ([[Mitsuhashi 1998|biblio/9769143]], [[Burnette and coll., 2014|biblio/25096480]]).
  - [[Marteijn and coll. (2003)|biblio/12474249]] optimised a culture medium using a genetic algorithm.  This methdod
    has been used at least once in invertebrates ([[Munroe and coll. (2019)|biblio/30747414]]).
  - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.