Café hier.

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+[[!meta title="Liquid drop of DNA libraries reveals total genome information."]]
+[[!tag emulsion PCR]]
+
+Terekhov SS, Eliseev IE, Ovchinnikova LA, Kabilov MR, Prjibelski AD, Tupikin AE, Smirnov IV, Belogurov AA Jr, Severinov KV, Lomakin YA, Altman S, Gabibov AG.
+
+Proc Natl Acad Sci U S A. 2020 Oct 21:202017138. doi:10.1073/pnas.2017138117.
+
+Liquid drop of DNA libraries reveals total genome information.
+
+[[!pmid 33087570 desc="“[...] either 3% of Abil EM 180 (A-oil) or a mixture of 4.5% Span 80/0.4% Tween 80/0.05% Triton X-100 (T-oil) was used [...] The homogeneity of the amplified DNA was much better in the case of A-oil, whereas ePCR in T-oil resulted in the formation of high-molecular-weight by-products [...] Thirty-five cycles of ePCR in A-oil were sufficient to reach saturation in the majority of the droplets. T-oil was less stable, displaying droplet coalescence after 25 cycles [...].”  “Appearance of clear amplicon bands on electropherograms does not correlate with the uniformity of amplification during ePCR.”  “ePCR clearly outperformed bulk PCR, reducing the number of reads with gross errors by twofold [and] resulted in a more uniform distribution of amplified sequences”"]]

diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
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+++ b/tags/emulsion.mdwn
@@ -21,6 +21,7 @@ Different kinds of surfactants:
  - Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid; primary paper: [[Kojima _et al._, 2005|biblio/16214800]])
  - DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]]
  - 3% fluorosurfactant (RAN Biotechnologies) in Novec HFE-7500 / 0.015% Tween 80 for double emulsions in [[Masted and coll., 2018|biblio/10.1101_409086]].
+ - 3% of Abil EM 180 or 4.5% Span 80/0.4% Tween 80/0.05% Triton X-100 were compared for ePCR by [[Terekhov and coll., 2020|biblio/33087570]]. “T-oil was less stable, displaying droplet coalescence after 25 cycles.”  “ePCR clearly outperformed bulk PCR, reducing the number of reads with gross errors by twofold [and] resulted in a more uniform distribution of amplified sequences”
 
 Reactive droplets: