[[!meta title="Reverse transcriptases can clamp together nucleic acids strands with two complementary bases at their 3'-termini for initiating DNA synthesis."]]
-[[!tag template_switching enzyme manganese]]
+[[!tag reverse_transcription template_switching enzyme manganese]]
Oz-Gleenberg I, Herschhorn A, Hizi A.
- [[T4 bacteriophage gene 32 protein|ssbp]] (T4gp32, [[Kenzelmann _et al._, 2004|biblio/15028277]],
[[Piché _et al._, 2005|biblio/16461948]]).
+
### DNA-dependent DNA polymerase activity
- It is utilised in [[template_switching]] methods to add linkers to first-strand cDNAs.
artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]).
- Its error profile is different from other DNA polymerases ([[de Paz et al., 2018|biblio/29718339]]).
+
+### Clamping activity
+
+ - Reverse transcriptases can bind duplexes that are only annealed through a
+ 2-nt homology. A single nucleotide is not enough and GC-rich sequences are
+ strongly favoured. This binding is enhanced by dGTP. The assays demonstrating
+ this used an ELISA approach ([[Oz-Gleenberg, Herschhorn and Hizi,
+ 2011|biblio/20876692]]).
+
+
### Terminal desoxynucleotidyl transferase (TdT) activity
Like other DNA polymerases ([[Clark, 1988|biblio/2460825]]), reverse