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+[[!meta title="Directed evolution of polymerase function by compartmentalized self-replication."]]
+[[!tag selection polymerase emulsion]]
+
+Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4552-7 doi:10.1073/pnas.071052198
+
+Ghadessy FJ, Ong JL, Holliger P.
+
+Directed evolution of polymerase function by compartmentalized self-replication.
+
+[[!pmid 11274352 desc="No beads. A bacterial cel is used as a subcompartment maintaining gene and proteins together. Evidence for leakage of small molecules at high compartment density."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index 1bfc85cd02c33467c723c4d594f3e3a8dbcdc3f2..e76008f652dc6420bba951aeca4808eeea2c943c 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -580,12 +580,6 @@ Only the constructs encoding methylases escape degradation.
 10471784 [emulsion]
 The gene product, fused to streptavidin, is bound to a biotinylated molecule in which it is encoded.
 
-12433997 [emulsion]
-Random primers were used to introduce mutations.
-
-11274352 [emulsion]
-No beads. A bacterial cel is used as a subcompartment maintaining gene and proteins together. Evidence for leakage of small molecules at high compartment density.
-
 16308152 [amplification]
 Detailed protocol for Terminal Continuation (TC).