single-strand (T)TTTGGG overhang, and prime second-strand synthesis.
- Cap-jumping ([[Efimov et al., 2001|biblio/11713326]]): oxidize diols,
- covalently bind a 3′-amine oligonucleotide. The [[reverse_transcriptase]]
- ireads through the cap structure and chemical bond, and integrates the reverse
+ covalently bind a 3′-amine oligonucleotide. The [[reverse transcriptase|reverse_transcription]]
+ reads through the cap structure and chemical bond, and integrates the reverse
complement of the oligonucleotide to the first-strand cDNA.
- [[Cleptet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4