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+[[!meta title="Amine-modified random primers to label probes for DNA microarrays."]]
+[[!tag microarray amplification method]]
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+Xiang CC, Kozhich OA, Chen M, Inman JM, Phan QN, Chen Y, Brownstein MJ.
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+Nat Biotechnol. 2002 Jul;20(7):738-42.
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+Amine-modified random primers to label probes for DNA microarrays.
+
+[[!pmid desc="Incorporates aminoallyl-dUTP instead of cy3/5dUTP. Uses priming hexamers with a amino C6dT in 5′. This allows to reduce by 10 folds the quantity of RNA as a starting material. Presence of amplification products of t/rRNAs is not a problem when « optimal » quantities of total RNA are used."]]
11687821
Review citing the 2 upper articles
-12089562 [amplification]
-* Incorporates aminoallyl-dUTP instead of cy3/5dUTP
-* uses priming hexamers with a amino C6dT in 5'
-This allows to reduce by 10 folds the quantity of RNA as a starting material.
-* Presence of amplification products of t/rRNAs is not a problem when «optimal» quantities of total RNA are used.
-
12172558
* Says that exponential (65+25 cycles) is more faithful than linear.
* Limits [dNTP] to produce only 3' cDNA that are a few hundrer base pairs long.