Centromeres.

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+[[!meta title="Quantitative analysis of centromeric FISH spots during the cell cycle by image cytometry."]]
+[[!tag cell_cycle centromere microscopy]]
+
+Amakawa G, Ikemoto K, Ito H, Furuya T, Sasaki K.
+
+J Histochem Cytochem. 2013 Oct;61(10):699-705. doi:10.1369/0022155413498754
+
+Quantitative analysis of centromeric FISH spots during the cell cycle by image cytometry.
+
+[[!pmid 23832878 desc="Fluorescence in situ hybridization (FISH) using chromosome enumeration DNA probes (CEPs) labeling centromeric regions.  The intensity of the staining increases as the cell progresses in the cell cycle.  On the published pictures, it is rare to be able to resolve the centromeric regions of the sister chromatids."]]

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 _Work in progress_
 
+## Sequence
+
  - Brute-force analysis to find the most abundant large tandem repeat can find
    centromeres ([[Melters and coll., 2013|biblio/23363705]]).
- - In medaka, study of homologous pairs of centromeres suggest that the
-   acrocentric ones evolve slower ([[Ichikawa and coll., 2017|biblio/29184138]]).
+ - In the three-spine stickleback, the centromere sequence of chrX differs from
+   the one of chrY ([[Peichel and coll., 2020|biblio/32684159]]).
+
+## Visualisation
+
  - In Oikopleura (and many others) H3S28p marks mitotic centromeres [[Fent and
    coll., 2019|biblio/31306061]].
+ - Centromeric regions of sister chromatids are rarely resolved in FISH.  Instead,
+   the intensity of the spot increases ([[Amakawa and coll., 2013|biblio/23832878]]).
+
+## Evolution
+
+ - In medaka, study of homologous pairs of centromeres suggest that the
+   acrocentric ones evolve slower ([[Ichikawa and coll., 2017|biblio/29184138]]).
  - Centromere breakage and inactivation in yeast: [[Sankaranarayanan and coll.,
    2020|biblio/31958060]].
  - Centromere relocation to a transcribed region in yeast, detected by ChIP of
    centromeric H3 [[Ola and coll., 2020|biblio/32424070]].
- - In the three-spine stickleback, the centromere sequence of chrX differs from
-   the one of chrY ([[Peichel and coll., 2020|biblio/32684159]]).
 
 [[!inline pages="tagged(centromere)" limit="0"]]