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+[[!meta title="Rapid Oocyte Growth and Artificial Fertilization of the Larvaceans Oikopleura dioica and Oikopleura longicauda"]]
+[[!tag Oikopleura]]
+
+Atsuo Nishino, and Masaaki Morisawa
+
+ZOOLOGICAL SCIENCE 15: 723–727 (1998) doi:10.2108/zsj.15.723
+
+Rapid Oocyte Growth and Artificial Fertilization of the Larvaceans Oikopleura dioica and Oikopleura longicauda
+
+[[!doi 10.2108/zsj.15.723 desc="Oikopleura (dioica and longicauda) sampled from Moroiso bay, Misaki, Miura peninsula and cultivated at 18 °C.  In O. dioica, the first polar body is visible 5 min after fertilisation and the second polar body 15 min after fertilisation.  O. longicauda individuals can self-fertilise.  F. pellucida could not be in vitro fertilised with the same method."]]

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
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--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -285,6 +285,8 @@ Tools
 Development
 -----------
 
+ - The first and second polar bodies are visible 5 and 15 min after fertilisation,
+   respectively ([[Nishino and Morisawa, 1998|biblio/10.2108_zsj.15.723]]).
  - First embryonic cleavages are deterministic and “Clonal organization of the
    tissues is essentially invariant among individuals” ([[Stach and coll.,
    2008|biblio/18490654]]).
@@ -471,6 +473,8 @@ Ecology
    (1980)|biblio/10069_30542]].
  - It was already reported to be frequent in Japanese waters [[in 1907 by T.
    Aida|biblio/AA0069577]].
+ - _O. dioica_, _longicauda_ and _cophocerca_ April 1997 in Moroiso Bay,
+   Misaki, Miura-peninsula, Kanagawa ([[Nishino and Morisawa, 1998|biblio/10.2108_zsj.15.723]]).
  - Oikopleuridae can be found in the deep see.  For instance, [[Lindsay and
    coll., 2014|biblio/10.1007_978-4-431-54865-2_51]] reported _Oikopleura_, _Mesochordaeus_
    and _Bathochordaeus_ individuals in the Hatoma Knoll hydrothermal vent, Okinawa Trough.