mraw

diff --git a/tags/template_switching.mdwn b/tags/template_switching.mdwn
index e10cf4a5a75c8c8899523a0717ae1caa594df421..e7f8577af1562872091ff195222a20a710d4a66a 100644 (file)
--- a/tags/template_switching.mdwn
+++ b/tags/template_switching.mdwn
@@ -21,6 +21,12 @@
    can extend a linker with the sequence of a small RNA via a template switching
    reaction.  (That is: a sRNA can play the same role as a TS oligonucleotide.)
 
+ - in _Capture and Amplification by Tailing and Switching_ (CATS,
+   [[Turchinovich et al (2014)|biblio/24922482]]), short and long RNAs are A-tailed,
+   oligo-dT-primed, and template swiched.  A PNK treatement is needed on
+   circulating RNAs, to remove phosphates or cyclophosphates that would
+   prevent the A-tailing.
+
 ### Effect of chemical composition of the TS oligonucleotide
 
 Originally, the TSOs were all-RNA.  Since this is expensive to synthesise,
@@ -38,6 +44,9 @@ as a replacement for RNA.
  - [[Arguel et al (2017)|biblio/27940562]] reported similar performance for
    RRR and RRL, using a 5′-focused method similar to nanoCAGE or STRT.
 
+ - 3′ phosphate or biotin blocking groups abolish template-switching
+   ([[Turchinovich et al (2014)|biblio/24922482]] and others).
+
 ### Effect of TSO concentration
 
  - For the STRT method, [[Zajac et al (2013)|biblio/24392002]] concluded that