Lu il y a longtemps.

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+[[!meta title="Application of an RNA amplification method for reliable single-cell transcriptome analysis."]]
+[[!tag single_cell method purification]]
+
+Biotechniques. 2015 Sep 1;59(3):137-48. doi:10.2144/000114331
+
+Suslov O, Silver DJ, Siebzehnrubl FA, Orjalo A, Ptitsyn A, Steindler DA.
+
+Application of an RNA amplification method for reliable single-cell transcriptome analysis.
+
+[[!pmid 26345506 desc="Purification by doing nothing: “We observed a substantial loss of template after the PCI procedure; however, we did notice that the inhibition was greatly diminished when RT tubes were simply stored at 40°C for 2–3 days without any cDNA purification. Therefore, we utilized this approach to reduce PCR inhibition.”"]]