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diff --git a/biblio/31114914.mdwn b/biblio/31114914.mdwn
new file mode 100644 (file)
index 0000000..6bc84b1
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+++ b/biblio/31114914.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging."]]
+[[!tag method ligase]]
+
+Nucleic Acids Res. 2019 May 22. pii: gkz435. doi:10.1093/nar/gkz435
+
+Miura F, Shibata Y, Miura M, Sangatsuda Y, Hisano O, Araki H, Ito T.
+
+Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging.
+
+[[!pmid desc="Add a A-tail with TdT before ligation, because ligase prefers As."]]

diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn
index c05a18a6190584715885d64be4ef403e36b95d6f..527c63a3b34ad3c11a0b6dd6e9dd657b4a3d5c3a 100644 (file)
--- a/tags/ligase.mdwn
+++ b/tags/ligase.mdwn
@@ -29,7 +29,7 @@ On T4 RNL2:
    linker to RNA ends, thus prevents unwanted ligation products
    ([[Viollet et al., 2011|biblio/21722378]]).
 
-DNA and RNA ligases can be used to adenylylate a substrate.
+DNA and RNA ligases can be used to [[adenylylate|adenylylation]] a substrate.
 
  - [[McLaughlin, Piel and Graeser (1985)|biblio/3978074]] noted that
    adenylylated RNA is a better substrate for T4 RNA ligase, and that
@@ -60,8 +60,13 @@ DNA and RNA ligases can be used to adenylylate a substrate.
    better tolerated with higher GC content and lower temperature.  Surprisingly,
    TNA overhangs were especially hard to ligate.
 
-[[!inline pages="tagged(ligase)" limit=0]]
-[[!meta title="pages tagged adenylylation"]]
+## Methods using ligases:
+
+(works continuously in progress)
 
+ - TACS (terminal deoxyribonucleotidyl transferase (TdT)-assisted adenylate
+   connector-mediated ssDNA) tails DNA with As using the TdT, in order to
+   benefit from the ligase's preference for As ([[Miura and coll.,
+   2019|biblio/31114914]]).
 
-[[!inline pages="tagged(adenylylation)" limit=0]]
+[[!inline pages="tagged(ligase)" limit=0]]