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diff --git a/biblio/10.1101_2020.09.08.286724.mdwn b/biblio/10.1101_2020.09.08.286724.mdwn
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-[[!meta title="Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis"]]
-[[!tag bioRxiv cell_culture karyotype]]
-
-Yoshinobu Uno, Ryo Nozu, Itsuki Kiyatake, Nobuyuki Higashiguchi, Shuji Sodeyama, Kiyomi Murakumo, Keiichi Sato, Shigehiro Kuraku
-
-bioRxiv 2020.09.08.286724; doi: https://doi.org/10.1101/2020.09.08.286724
-
-Cell culture-based shark karyotyping as a resource for chromosome-scale genome analysis
-
-[[!doi 10.1101/2020.09.08.286724 desc="Primary cell culture of fibroblasts and lymphocytes.  Mitogens were used to stimulate lymphocyte growth. “2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum)”.  In comparison with teleost cell culture medium, shark cells needed a higher osmolarity and the medium was supplemented with 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide."]]

diff --git a/biblio/33159152.mdwn b/biblio/33159152.mdwn
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+[[!meta title="Cell culture-based karyotyping of orectolobiform sharks for chromosome-scale genome analysis."]]
+[[!tag cell_culture karyotype]]
+
+Yoshinobu Uno, Ryo Nozu, Itsuki Kiyatake, Nobuyuki Higashiguchi, Shuji Sodeyama, Kiyomi Murakumo, Keiichi Sato, Shigehiro Kuraku
+
+Commun Biol. 2020 Nov 6;3(1):652. doi:10.1038/s42003-020-01373-7
+
+Cell culture-based karyotyping of orectolobiform sharks for chromosome-scale genome analysis.
+
+[[!pmid 33159152 desc="Primary cell culture of fibroblasts and lymphocytes.  Mitogens were used to stimulate lymphocyte growth. “2n = 102 for the whale shark (Rhincodon typus) and zebra shark (Stegostoma fasciatum), and 2n = 106 for the brownbanded bamboo shark (Chiloscyllium punctatum) and whitespotted bamboo shark (C. plagiosum)”.  In comparison with teleost cell culture medium, shark cells needed a higher osmolarity and the medium was supplemented with 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide."]]

diff --git a/tags/cell_culture.mdwn b/tags/cell_culture.mdwn
index b387b0ce2cd66ad47a0839f1f7b432d8e113c0d8..f4a0970c212713446431f63be4acf7a58ead1477 100644 (file)
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+++ b/tags/cell_culture.mdwn
@@ -8,5 +8,6 @@ A few notes that just scratch the surface of a vast field…
  - [[Marteijn and coll. (2003)|biblio/12474249]] optimised a culture medium using a genetic algorithm.  This methdod
    has been used at least once in invertebrates ([[Munroe and coll. (2019)|biblio/30747414]]).
  - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.
+ - Shark cells need high osmolarity and a supplement of 333 mM urea, 188 mM NaCl and 54 mM trimethylamine N-oxide ([[Uno and coll., 2020|biblio/33159152]]).
 
 [[!inline pages="tagged(cell_culture)" limit=0]]