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diff --git a/biblio/14990785.mdwn b/biblio/14990785.mdwn
index 9b987ab9e433fc2e77c95c525856d3c064eb1df4..482deef460defdeba25be594c87fa94ab52b080f 100644 (file)
--- a/biblio/14990785.mdwn
+++ b/biblio/14990785.mdwn
@@ -1,3 +1,10 @@
 [[!meta title="A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system."]]
-[[!tag emulsion product not_read]]
-[[!pmid 14990785 desc="Uses ABIL EM90"]]
+[[!tag emulsion DTT]]
+
+Protein Eng Des Sel. 2004 Mar;17(3):201-4 doi:10.1093/protein/gzh025
+
+Ghadessy FJ, Holliger P.
+
+A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system.
+
+[[!pmid 14990785 desc="Primary paper for the use of ABIL EM90.  Span/Tween superfactants oxydise the reagents.  DTT partly prevents oxydisation but inhibits reactions above 20 mM."]]

diff --git a/tags/emulsion.mdwn b/tags/emulsion.mdwn
index bb76020d326b8f0fea78e51d13250847d3614b1d..41f66a5cbdf9e00e3b0ae3300a3eef4a33e6bf32 100644 (file)
--- a/tags/emulsion.mdwn
+++ b/tags/emulsion.mdwn
@@ -14,6 +14,10 @@ How to break the droplets ?
    - In [[Kojima _et al._, 2005|biblio/16214800]], by mixing with 1M NaCl, 5 mM Tris-HCl pH 8.0, 0.5 mM EDTA (oil: mineral; superfactant: Sun Soft No. 818SK).
  - Extraction of beads with hexane:oil 1:1, in [[Levy _et al._, 2005|biblio/16131588]] (oil: mineral, superfactant: 5.5% Span 80, 0.5% Tween 80, 0.1 % TritonX-100).
 
-Different kinds of superfactants: TritonX-100, Span 80, Tween 80 (many articles), ABIL WE09 ([[Dielh _et al._, 2006|biblio/16791214]] and others), Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid [[Kojima _et al._, 2005|biblio/16214800]]), DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]].
+Different kinds of superfactants:
+ - TritonX-100, Span 80, Tween 80 (many articles),
+ - ABIL WE09 (polysiloxane–polycetyl–polyethylene glycol copolymer; primary paper: [[Dielh _et al._, 2004|biblio/14990785]])
+ - Sun Soft No. 818SK (polyglycerol esters of intersesterified ricinoleic acid; primary paper: [[Kojima _et al._, 2005|biblio/16214800]])
+ - DC 5225C Formulation Aid / DC 749 Fluid (Dow Chemical Co.) [[Margulies _et al._, 2005|biblio/16056220]]
 
 [[!inline pages="tagged(emulsion)" actions="no" feedshow=0]]