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+[[!meta title="Quantitative analysis of mRNA amplification by in vitro transcription."]]
+[[!tag ssbp reverse_transcription amplification]]
+
+Nucleic Acids Res. 2001 Mar 1;29(5):E29
+
+Baugh LR, Hill AA, Brown EL, Hunter CP.
+
+Quantitative analysis of mRNA amplification by in vitro transcription.
+
+[[!pmid 11222780 desc="T7 pol generates aberrant template-unrelated products. This is fixed by reducing the concentration of oligo dT primers, and of enzymes. Under these conditions, 2 rounds of RNA linear amplification is not generatng too much bias. Small-volume protocol. T4gp32, a single stranded protein, increases RT processivity."]]

diff --git a/biblio/To_Do b/biblio/To_Do
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--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -38,9 +38,6 @@ Noise due to RNA extraction is 40 times greater than noise due to replicate hybr
 12595569 [amplificaiton]
 Aminoallyl UTP to labal cRNAs. In contrast to cDNAs, DMSO is required for subsequent cyanine labelling.
 
-11222780 [amplification]
-T7 pol generates aberrant template-unrelated products. This is fixed by reducing the concentration of oligo dT primers, and of enzymes. Under these conditions, 2 rounds of RNA linear amplification is not generatng too much bias. Small-volume protocol. T4gp32, a single stranded protein, increases RT processivity.
-
 12161654 [misc]
 Fluorescent bar-coded oligos to monitor transcription in vivo.