Ligation to DNA.

diff --git a/biblio/14967495.mdwn b/biblio/14967495.mdwn
index bc7e2e5356d0e85d658470556436b9ff7e7f126f..2cea2745f2cd1b2a2e420f751f3da4815938369e 100644 (file)
--- a/biblio/14967495.mdwn
+++ b/biblio/14967495.mdwn
@@ -7,4 +7,5 @@ Yin S, Kiong Ho C, Miller ES, Shuman S.
 
 Characterization of bacteriophage KVP40 and T4 RNA ligase 2.
 
-[[!pmid 14967495 desc="One more tool in the box?"]]
+[[!pmid 14967495 desc="One more tool in the box? Ligation of RNA to an acceptor
+where the last base is DNA is stronlgy suppressed."]]

diff --git a/tags/ligase.mdwn b/tags/ligase.mdwn
index 2490e339b577042a9c32a9a5133cdbeab4a85ba2..d455a1610eb53f6262277b3808c6fa33e0f62b49 100644 (file)
--- a/tags/ligase.mdwn
+++ b/tags/ligase.mdwn
@@ -6,6 +6,11 @@ On T4 RNL2:
 
  - Functional residues analysed by [[Yin et al., 2003|biblio/12611899]].
  - The truncated Rnl2 was published by [[Ho et al., 2004|biblio/14962393]].
+ - Ligation of RNA to a DNA acceptor is very weak ([[Yin et al., 2004|biblio/14967495]].
+ - The RNA acceptor strand participates to its ligation by promoting
+   adenylylation of the donor, and by promoting the formation of the
+   phosphodiester bond.  With DNA, this step is 35 times slower
+   ([[Nandakumar et al., 2005|biblio/15851476]]).
  - The K227Q mutation prevents transfer of the andenylyl residue from the
    linker to RNA ends, thus prevents unwanted ligation products. 
    [[Viollet et al., 2011|biblio/21722378]]