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+[[!meta title="Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein."]]
+[[!tag amplification method ssbp]]
+
+Inoue J, Shigemori Y, Mikawa T.
+
+Nucleic Acids Res. 2006 May 17;34(9):e69 doi:10.1093/nar/gkl350
+
+Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein.
+
+[[!pmid 16707659 desc="By weakening the DNA-protein interaction, the authors acheived the suppression of the non-temlated products in rolling circle amplification."]]
16682450 [misc]
The structure of the genetic code favours secondary structures in coding RNA.
-16707659 [enzymes]
-By weakening the DNA-protein interaction, the authors acheived the suppression of the non-temlated products in rolling circle amplification.
-
16618967 [enzymes]
The combined use of a complementary DNA oligo and DNA ligase suppresses the artifacts seen in RNA ligase adenylation.
- "Not-so random" (NSR) primers: [[Armour et al., 2009|biblio/19668204]].
- ... and many more (pseudo-random, ...)
+[[Single-strand binding proteins|ssbp]] are sometimes added to improve reverse
+transcriptions.
+
[[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]]
[[!meta title="pages tagged ssbp"]]
-[[!inline pages="tagged(ssbp)" actions="no" archive="yes"
-feedshow=10]]
+Single-strand binding proteins
+==============================
+
+(Often used as additives in molecular biology reactions.)
+
+[[!inline pages="tagged(ssbp)" actions="no" limit=0]]