Read on Tuesday.

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+[[!meta title="Four methods of preparing mRNA 5' end libraries using the illumina sequencing platform."]]
+[[!tag template-switching method oligo-capping benchmark]]
+
+Machida RJ, Lin YY.
+
+PLoS One. 2014 Jul 8;9(7):e101812. doi: 10.1371/journal.pone.0101812
+
+Four methods of preparing mRNA 5' end libraries using the illumina sequencing platform.
+
+[[!pmid 25003736 desc="Capture of 5′ ends via biotinylated forward PCR primers.  CapSMART: ligation of modified oligonucleotides that block RT on 5′-phoshphorylated RNAs (after kinase treatment).  The signal is not much different from standard SMART."]]