Le soir.

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+[[!meta title="Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer."]]
+[[!tag cellulose Oikopleura]]
+
+Li KL, Nakashima K, Inoue J, Satoh N.
+
+Genes (Basel). 2020 Aug 13;11(8):937. doi:10.3390/genes11080937
+
+Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer.
+
+[[!pmid 32823766 desc="Comparison of primary sequence and of intron position suggests that GHC6-1 was transferred in a common ancestor of all living tunicates.  _O. dioica_'s GH6-1 gene lacks a conserved catalytic aspartate, and does not share introns with the other tunicate genes."]]
+
+“We found that in many tunicate GH6-1 proteins, an aspartic acid can be aligned to the catalytic _H. jecorina_ D221, except for SthGH6-1b (E197) and OdiGH6-1 (K211).  However, the catalytic aspartic acid was not conserved in tunicate CesA.”
+
+“In this study, although we found no other sites shared between genes of _O. dioica_ and other tunicates, we found that many shared splice sites are present among GH6-containing genes from three other major clades of tunicates (Thaliacea + Phlebobranchia + Stolidobranchia). It is reasonable to assume that many shared introns were acquired after the branching of larvaceans and before the subsequent divergence of major tunicate clades.”

diff --git a/tags/Oikopleura.mdwn b/tags/Oikopleura.mdwn
index 9475b5a308caf985f9bc1b634c2af73701ac5921..1684c65ff6621e4e46ee37d0aca37b10ef9ac5b7 100644 (file)
--- a/tags/Oikopleura.mdwn
+++ b/tags/Oikopleura.mdwn
@@ -202,7 +202,9 @@ Genes and pathways
    intestinalis_ ([[Matthysse and coll., 2004|biblio/14722352]]).
  - A GH6 (glycosyl hydrolase family 6) domain is found in the CesA genes,
    as well as an independent genes, in Tunicates
-   ([[Inoue, Nakashima and Satoh, 2019|biblio/30974905]]).
+   ([[Inoue, Nakashima and Satoh, 2019|biblio/30974905]]).  In _Oikopleura_, it
+   lacks a conserved catalytic aspatate, and does not share ancestral introns with
+   other tunicates ([[Li and coll., 2020|biblio/32823766]]).
  - _O. dioica_ has multiple CDK1 and Cyclin B paraplogs, some of which are
    implicated in oogenesis ([[Øvrebø and coll., 2015|biblio/25714331]],
    [[Feng & Thompson, 2018|biblio/29969934]]).