Café

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+[[!meta title="Metabarcoding analysis of trophic sources and linkages in the plankton community of the Kuroshio and neighboring waters."]]
+[[!tag eDNA]]
+
+Kobari T, Tokumo Y, Sato I, Kume G, Hirai J.
+
+Sci Rep. 2021 Dec 1;11(1):23265 doi:10.1038/s41598-021-02083-8
+
+Metabarcoding analysis of trophic sources and linkages in the plankton community of the Kuroshio and neighboring waters.
+
+[[!pmid 34853330 desc="eDNA analysis of “the diets of 22 higher taxonomic groups, 13 copepod families and 35 species, that dominate in the Kuroshio food web”.  Larvacean DNA was more frequent in predator gut contents than in water samples.  “The 18S rRNA V9 region was amplified using eukaryotic universal primers 1389F and 1510R.”"]]

diff --git a/tags/eDNA.mdwn b/tags/eDNA.mdwn
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  - Edgar ([[2018|biblio/29506021]] proposes to raise the threshold to 99% or to
    just use sequence variants (also called ZOTUS, Zero-radius OTUs. 
 
+ - Larvacean DNA found more frequently in predator gut than in water samples
+   ([[Kobari and coll., 2021|biblio/34853330]]).  Uses the V9 region 18S primers.
+
 ### Published primers
 
 ```