low-complexity “pseudo-random” reverse-transcription primers ([Arnaud and
coll., 2016](https://pubmed.gov/27071605)).
-On April 2013, I started a new development cycle as the leader of the
-Genomics Miniaturization Technology Unit at RIKEN Center for Life
-Sciences, Division of Genomics Technology, to expand this work on
-single cells following a **population transcriptomics** approach (Plessy
-et al., 2013) focused on sampling the largest possible number of
-cells. In our ongoing developments, we have reached **single-cell and
+On April 2013, I started a new development cycle as the leader of the Genomics
+Miniaturization Technology Unit at RIKEN Center for Life Sciences, Division of
+Genomics Technology, to expand this work on single cells following a
+**population transcriptomics** approach ([Plessy and coll.,
+2013](https://pubmed.gov/23281054)) focused on sampling the largest possible
+number of cells. In our ongoing developments, we have reached **single-cell and
single molecule resolution** through the introduction of transposase
-fragmentation and unique molecular identifiers (Poulain et al.,
-2017). The protocol exists in two versions, one for FACS-isolated
-cells, and one for the Fluidigm C1 platform (Kouno et al., 2019).
+fragmentation and unique molecular identifiers ([Poulain and coll.,
+2017](https://pubmed.gov/28349422)). The protocol exists in two versions, one
+for FACS-isolated cells, and one for the Fluidigm C1 platform ([Kouno and coll.,
+2019](https://pubmed.gov/30664627)).
-I have complemented my work on CAGE with the development of a
-gene-centred technique for detecting promoters, termed Deep-RACE
-(Olivarius et al., 2009, Plessy et al., 2012), which we used to
-validate our discovery of the pervasive expression of retrotransposons
-detected by CAGE (Faulkner et al., 2009). To study transcription start
-activity at nucleotide resolution in zebrafish transfected with
-chimeric transgenes containing a copy of an endogenous promoter, I
-combined Deep-RACE, CAGE and paired-end sequencing in a technology
-that we called “Single-Locus CAGE” (Haberle et al., 2014). With my
-contributions related to CAGE development and analysis, I have been a
-**member of the FANTOM consortium** since FANTOM3.
+I have complemented my work on CAGE with the development of a gene-centred
+technique for detecting promoters, termed Deep-RACE ([Olivarius and coll.,
+2009](https://pubmed.gov/19317658), [Plessy and coll.,
+2012](http://dx.doi.org/10.1002/9783527644582.ch4)), which we used to validate
+our discovery of the pervasive expression of retrotransposons detected by CAGE
+([Faulkner and coll., 2009](https://pubmed.gov/19377475)). To study
+transcription start activity at nucleotide resolution in zebrafish transfected
+with chimeric transgenes containing a copy of an endogenous promoter, I
+combined Deep-RACE, CAGE and paired-end sequencing in a technology that we
+called “Single-Locus CAGE” ([Haberle and coll.,
+2014](https://pubmed.gov/24531765)). With my contributions related to CAGE
+development and analysis, I have been a **member of the FANTOM consortium**
+since FANTOM3.
Together with my colleagues at RIKEN and collaborators in the field of
neuroscience, I have applied nanoCAGE to the study of single neuron
-cell types, for instance the **olfactory neurons** (Plessy et al., 2012),
+cell types, for instance the **olfactory neurons** ([Plessy et al., 2012),
or in dopaminergic cells, where we could demonstrate the expression of
haemoglobin in the midbrain (Biagioli et al., 2009). We are also
exploring the sub-cellular localisation of RNA in **Purkinje neurons**