Evolution of the U2 Spliceosome for Processing Numerous and Highly Diverse Non-canonical Introns in the Chordate Fritillaria borealis.
-[[!pmid 31543449 desc="In Fritillaria borealis, most ancestral canonical
-introns were lost. New introns with non-canonical splice sites were created by
+[[!pmid 31543449 desc="In Fritillaria borealis, most ancestral GT/AG canonical
+introns were lost. New AG/AR introns with non-canonical splice sites were created by
the insertion of MITEs (miniature inverted-repeat transposable elements) after
TA sites. Splicing of non-canonical introns can be inhibited with Pladienolide
B. Non-canonical introns of F. bor and O. dioica genes can not be spliced in
- Analysis of sex-linked markers supports genetic sex determination with male heterogamety –
that is: X chromosomes for females and Y for males. ([[Denoeud et al., 2010|biblio/21097902]])
- The major spliceosome is hypothethised to have evolved
- to become more permissive in order to splice G*-AG sites. ”A large fraction
+ to become more permissive in order to splice G*/AG sites. ”A large fraction
(more than 10%) of the (...) introns displayed non-canonical (non GT-AG)
splice sites, whereas the usual proportion is around 1%-1.5% in other genomes”
([[Denoeud et al., 2010|biblio/21097902]]).
2010|biblio/21097902]]). Larger introns tend to be older, and among the large
introns, the older contain repeat elements less frequently than the newer
([[Denoeud et al., 2010|biblio/21097902]]). In _Fritilaria borealis_, most
- introns are derived from the insertion of MITE elements and are non-canonical
+ introns are derived from the insertion of MITE elements and are AG/AR non-canonical
([[Henriet and coll., 2019|biblio/31543449]]).
- - HEK293T cells can only splice canonical _O. dioica_ (and _F. borealis_) introns,
- but not the non-canonical ones ([[Henriet and coll., 2019|biblio/31543449]]).
+ - HEK293T cells can only splice canonical GT/AG _O. dioica_ (and _F. borealis_) introns,
+ but not the non-canonical AG/AR ones ([[Henriet and coll., 2019|biblio/31543449]]).
- 12 developmental stages were studied with tiling arrays by [[Danks et al., 2013|biblio/23185044]].
- On the histone mRNAs, no purine-rich histone downstream element (HDE) was found
by [[Chioda, Eskeland and Thompson in 2002|biblio/12446815]].