Polyamines.

diff --git a/tags/cell_culture.mdwn b/tags/cell_culture.mdwn
index 25a0c4c3c4313c4ad6ad09bb2de6cdb11ac420ba..53cadb5725ddcf659923330288e1caa90d62889e 100644 (file)
--- a/tags/cell_culture.mdwn
+++ b/tags/cell_culture.mdwn
@@ -4,6 +4,7 @@ A few notes that just scratch the surface of a vast field…
 
  - [[Echalier and Ohanessian (1969)|biblio/4976834]] reported a culture of _Drosophila_ cells that spontaneously transformed.
  - [[Schneider's (1972)|biblio/4625067]] report of the establishment of the S2 cell line.
+ - Polyamines are needed for the long-term passaging of some cell lines ([[Mitsuhashi 1998|biblio/9769143]]).
  - [[Marteijn and coll. (2003)|biblio/12474249]] optimised a culture medium using a genetic algorithm.  This methdod
    has been used at least once in invertebrates ([[Munroe and coll. (2019)|biblio/30747414]]).
  - [[Kawamura and coll. (2021)|biblio/33899125]] reported the use of plasmin to establish coral cell lines.