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+[[!meta title="NanoPARE: parallel analysis of RNA 5' ends from low-input RNA."]]
+[[!tag RNA-seq sequence_tags method library template_switching software]]
+
+Genome Res. 2018 Dec;28(12):1931-1942. doi:10.1101/gr.239202.118
+
+Schon MA, Kellner MJ, Plotnikova A, Hofmann F, Nodine MD.
+
+NanoPARE: parallel analysis of RNA 5' ends from low-input RNA.
+
+[[!pmid 30355603 desc="5′ ends tags and RNA-seq tags separately amplified in two libraries."]]

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    circulating RNAs, to remove phosphates or cyclophosphates that would
    prevent the A-tailing.
 
+ - in _nanoPARE_ ([[Schon, Kellner and coll.|biblio/30355603]]), a template-switching
+   oligonucleotide (RNA-RNA-LNA, without UMIs) is used to add a linker on 5′ ends.
+   After tagmentation, two libraries are amplified: one for 5′ ends and one for RNA-seq.
+   ~15% of the 5′ end alignments have extra Gs, but the genomic distribution is
+   bimodal.  Peaks with significant amounts of "extra G" nucleotides are marked as TSS.
+
 ### Effect of chemical composition of the TS oligonucleotide
 
 Originally, the TSOs were all-RNA.  Since this is expensive to synthesise,