Old

diff --git a/biblio/15272081.mdwn b/biblio/15272081.mdwn
new file mode 100644 (file)
index 0000000..95a7d69
--- /dev/null
+++ b/biblio/15272081.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="5' Long serial analysis of gene expression (LongSAGE) and 3' LongSAGE for transcriptome characterization and genome annotation."]]
+[[!tag sequence_tags library]]
+
+Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11701-6. doi:10.1073/pnas.0403514101
+
+Wei CL, Ng P, Chiu KP, Wong CH, Ang CC, Lipovich L, Liu ET, Ruan Y.
+
+5' Long serial analysis of gene expression (LongSAGE) and 3' LongSAGE for transcriptome characterization and genome annotation.
+
+[[!pmid 15272081 desc="81% of the 5'/3' tag pairs could be used to amplify a cDNA by RT-PCR."]]

diff --git a/biblio/To_Do b/biblio/To_Do
index 524dacc71c3cd9bd47677f20314757c7ee7760aa..88c9c5630d5abb06a151624b388aa328350c6cde 100644 (file)
--- a/biblio/To_Do
+++ b/biblio/To_Do
@@ -89,9 +89,6 @@ T4 DNA ligase used to ligate a double stranded cap-tag.
 3799962 [enzymes]
 Optimal : Donor/acceptor 5:1; hexammine cobalt chloride 5.0 mM, PEG8000 25%; ATP 100µM. Inhibitors: ATP > 100 µM, MN 2+ > 5mM, NaCl > 20 mM.
 
-15247329 [libraries] [tracked]
-Ligation of cDNA and methylated oligos, in presence of methy-sensitive restriction enzymes, eliminates the cDNA dimer product du to mass action.
-
 14513556 [libraries]
 Hybridises single-stranded tester plasmid and driver PCR product, then purified single strand molecules out of duplexes using hydroxyapatite.
 
@@ -101,9 +98,6 @@ A method to amplify two distinguishable substrates in the same PCR tube, so that
 12582258 [misc]
 Direct labeling of 10µg total RNA.
 
-15272081 [libraries]
-81% of the 5'/3' tag pairs could be used to amplify a cDNA by RT-PCR.
-
 8125298 [libraries]
 Oligo capping primary paper.