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diff --git a/biblio/12777618.mdwn b/biblio/12777618.mdwn
new file mode 100644 (file)
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+++ b/biblio/12777618.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype."]]
+[[!tag method cap library]]
+
+Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7033-8 doi:10.1073/pnas.1232347100
+
+Choi YH, Hagedorn CH.
+
+Purifying mRNAs with a high-affinity eIF4E mutant identifies the short 3' poly(A) end phenotype.
+
+[[!pmid 12777618 desc="Increases cap specificity with a K119A mutation.  This mutant is fused to GST instead of protein A."]]

diff --git a/tags/cap.mdwn b/tags/cap.mdwn
index e3333e7cd6ad174b71b34e5d0e67efe99cfea84c..afc78ea9ffb9d74bc75173a7a2ce34d43f9f76c4 100644 (file)
--- a/tags/cap.mdwn
+++ b/tags/cap.mdwn
@@ -33,6 +33,9 @@ Methods for enriching capped RNAs
    reads through the cap structure and chemical bond, and integrates the reverse
    complement of the oligonucleotide to the first-strand cDNA.
 
+ - [[Choi and Hagedorn (2003)|biblio/12777618]] improved the CAPture method by using
+   a K119A mutant of eIF4E.  It is fused to GST instead of protein A.
+
  - [[Clepet et al., 2004|biblio/14704363]] modified oligo-capping, to use T4
    DNA ligase and a double-stranded adapter with NNNNNN overhang instead of T4
    RNA ligase and a single-stranded linker.