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+[[!meta title="RT-PCR without RNA isolation."]]
+[[!tag RNA extraction RT-PCR DTT]]
+
+Klebe RJ, Grant GM, Grant AM, Garcia MA, Giambernardi TA, Taylor GP.
+
+Biotechniques. 1996 Dec;21(6):1094-100.
+
+RT-PCR without RNA isolation.
+
+[[!pmid 8969838 desc="Freeze-thaw method.  5 mM DTT partially protects the RNA for 1 h in absence of RNAse inhibitors.  Hypertonic solutions may prevent the premature disruption of the cell and its lysosomes prior lysis."]]