- Cleavage is impaired by proteins bound between the sites [[Meisel et al., 1995|biblio/7796821]].
- Positive bias for adenine stretches on the 5′ or 3′ side of CAGCAG [[Möncke-Buchner et al., 2009|biblio/19250940]].
- Stimulated by AdoMet and sinefungin [[Raghavendra & Rao, 2005|biblio/16026759]].
+ - Single-site cleavage is possible with higher concentration of enzyme ([[Ahmad and co., 2018|biblio/29846668]]).
Used in [[SuperSAGE|biblio/14676315]], [[HELP-tagging|biblio/20359321]], [[nanoCAGE|biblio/20543846]], [[CAGE|biblio/22362160]]. (non-exhaustive list)