When coverage is too low for efficient reference-free assembly, related
references can be used as a guide. The Ragout software ([[Kolmogorov and
coll., 2014|biblio/24931998]], [[Kolmogorov and coll., 2018|biblio/30341161]])
-can take multiple reference genomes to guide the assembly of one target.
-Polymorphisms unique to the target genome can be recovered, but chromosome
-fusions are typically hard to detect. Compared to version 1, version 2 infers
-phylogenetic relationships between the reference genomes automatically. An
-alterative reference-guided assembler, RagOO ([[Alonge and coll.,
-2019|biblio/31661016]]) is reported to be faster, but can only take a single
-reference.
+can take multiple reference genomes to guide the scaffolding of a target
+assembly. Polymorphisms unique to the target genome can be recovered, but
+chromosome fusions are typically hard to detect. Compared to version 1,
+version 2 infers phylogenetic relationships between the reference genomes
+automatically. An alterative reference-guided scaffolder, RagOO ([[Alonge and
+coll., 2019|biblio/31661016]]) is reported to be faster, but can only take a
+single reference.
The HaploMerger2 pipeline ([[Huang and coll., 2017|biblio/28407147]]) takes a
diploid assembly and outputs a reference and an alternative sub-assembly for