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+[[!meta title="Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase."]]
+[[!tag reverse_transcriptase]]
+
+Science. 1992 Nov 13;258(5085):1112-8 doi:10.1126/science.1279806
+
+Peliska JA, Benkovic SJ.
+
+Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
+
+[[!pmid 1279806 desc="Addition of 1 extra nucleotide happens at an apparent rate of 0.1 / min.  Addition of subsequent bases is visible (Fig 3) but much slower.  This addition does not requrie RNAseH activity.  A > G > T > C ratio on a non-capped oligonucleotide duplex: 1 : 0.5 : 0.2 : 0.07, determined by incubating the reaction with each individual dNTP.  50 mM Tris-HCl pH 8, 75 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 200 nM DNA/RNA duplex, each dNTP at 100 µM, 7 mM MgCl2, 800 nM TSO and 200 nM HIV-1 RT, at 37 °C."]]

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
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 Like other DNA polymerases ([[Clark, 1988|biblio/2460825]]), reverse
 transcriptases have a TdT activity.
 
+Peliska JA, Benkovic SJ.
+
+Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase.
+
+ - [[Peliska and Benkovic, 1992|biblio/1279806]] showed that HIV-1 RT adds one
+   nucleotide on non-capped oligonucleotide duplexes.   A > G > T > C ratio:
+   1 : 0.5 : 0.2 : 0.07.  Addition of subsequent bases is visible but much
+   slower.
+
  - [[Patel & Preston, 1994|biblio/7507249]] showed that HIV RT adds one
    nucleotide (A > G >> T|C) on DNA/DNA duplexes, and more on DNA/RNA duplexes.
    Addition is favoured by increased dNTP levels.  MMLV and AMV were also