+++ /dev/null
-[[!meta title="HiCanu: accurate assembly of segmental duplications, satellites, and allelic variants from high-fidelity long reads"]]
-[[!tag bioRxiv genome assembly chromosome]]
-
-Sergey Nurk, Brian P Walenz, Arang Rhie, Mitchell R Vollger, Glennis A Logsdon, Robert Grothe, Karen H Miga, Evan E Eichler, Adam M Phillippy, Sergey Koren
-
-bioRxiv 2020.03.14.992248; doi: https://doi.org/10.1101/2020.03.14.992248
-
-HiCanu: accurate assembly of segmental duplications, satellites, and allelic variants from high-fidelity long reads
-
-[[!doi 10.1101/2020.03.14.992248v3 desc="“HiCanu modifies the input reads by
-compressing every homopolymer to a single nucleotide.” “Outputs contigs as
-“pseudo-haplotypes” that preserve local allelic phasing but may switch between
-haplotypes”"]]
--- /dev/null
+[[!meta title="HiCanu: accurate assembly of segmental duplications, satellites, and allelic variants from high-fidelity long reads"]]
+[[!tag genome assembly chromosome]]
+
+Sergey Nurk, Brian P Walenz, Arang Rhie, Mitchell R Vollger, Glennis A Logsdon, Robert Grothe, Karen H Miga, Evan E Eichler, Adam M Phillippy, Sergey Koren
+
+Genome Res. 2020 Sep;30(9):1291-1305. doi:10.1101/gr.263566.120
+
+HiCanu: accurate assembly of segmental duplications, satellites, and allelic variants from high-fidelity long reads
+
+[[!pmid 32801147 desc="“HiCanu modifies the input reads by
+compressing every homopolymer to a single nucleotide.” “Outputs contigs as
+“pseudo-haplotypes” that preserve local allelic phasing but may switch between
+haplotypes”"]]
with the reference. Shasta also comes with polishing modules similar to Racon
and Medaka, but also to be faster.
+The HiCanu assembler ([[Nurk, Walenz and coll., 2020|biblio/32801147]]) can
+take advantage of high-accuracy sequences such as the ones of the PacBio HiFi
+platform, to assemble multiple variants of the same locus.
+
Some genome assemblers produce a graph file that can be visualised
with tools such as Bandage [[Wick and coll., 2015|biblio/26099265]].