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diff --git a/biblio/20716356.mdwn b/biblio/20716356.mdwn
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+++ b/biblio/20716356.mdwn
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+[[!meta title="Multi-targeted priming for genome-wide gene expression assays."]]
+[[!tag random_priming method]]
+
+BMC Genomics. 2010 Aug 17;11:477. doi:10.1186/1471-2164-11-477
+
+Adomas AB, Lopez-Giraldez F, Clark TA, Wang Z, Townsend JP.
+
+Multi-targeted priming for genome-wide gene expression assays.
+
+[[!pmid 20716356 desc="NDKTBBBBDWGS occurs one or more times in 76% of 6608 mRNAs present in budding yeast, but is absent in rRNA or tRNA."]]

diff --git a/biblio/9973624.mdwn b/biblio/9973624.mdwn
index af76a8489fbc70a2d4bbb7ad1d1ecce7eca9527e..a2f3f88099948f8f4848a5c127d8cdcb91d8186e 100644 (file)
--- a/biblio/9973624.mdwn
+++ b/biblio/9973624.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Increased specificity of reverse transcription priming by trehalose and oligo-blockers allows high-efficiency window separation of mRNA display."]]
-[[!tag thermo-enhancement enzyme random_priming]]
+[[!tag thermo-enhancement enzyme random_priming reverse_transcription]]
 
 Mizuno Y, Carninci P, Okazaki Y, Tateno M, Kawai J, Amanuma H, Muramatsu M, Hayashizaki Y.
 

diff --git a/tags/reverse_transcription.mdwn b/tags/reverse_transcription.mdwn
index 9d9ac9d8b2dd2e38b02404ec950ee5f5a61596a1..24fa500a513542c6e4bb3183cc743e35a718af1e 100644 (file)
--- a/tags/reverse_transcription.mdwn
+++ b/tags/reverse_transcription.mdwn
@@ -15,9 +15,13 @@ Reverse-transcriptases have a DNA-dependent DNA polymerase activity.
    but not RNA-dependent polymerase activity and is used to suppress these
    artefacts ([[Perocchi et al., 2007|biblio/17897965]], [[Kanamori-Katayama et al., 2011|biblio/21596820]]).
 
+Reverse-transcriptases tolerate terminal mismatches: [[Mizuno et al.,1999 |biblio/9973624]].
+
 Reverse-transcription primers:
 
  - "N15" random pentadecamers: [[Stangegaard et al., 2006|biblio/16708763]].
- - ... and many more (not-so-random; pseudo-random, ...)
+ - Multi-targeted primers (MTP): [[Adomas et al., 2010|biblio/20716356]].
+ - "Not-so random" (NSR) primers: [[Armour et al., 2009|biblio/19668204]].
+ - ... and many more (pseudo-random, ...)
 
 [[!inline pages="tagged(reverse_transcription)" actions="no" limit=0]]